[Functional E rosette study pre and post laser treatment in malignant ocular melanoma].

For 11 patients with ocular malignant melanoma (OMM) suspicion, before and after laser photocoagulation therapy, by E rosette test were studied. A higher activation and sensitisation to OMM antigen was obtained after laser therapy in 9 patients. These results suggest the influence of laser treatment on lymphocyte function by "revealing" the physiological reactivity.

Fibronectin and lymphocyte blast transformation.

Purified human plasma fibronectin (Fn) added to human peripheral blood leukocytes culture, from 22 healthy blood donors, phytohemagglutinin (PHA) stimulated, enhanced mitogen action in blast transformation. Daily measurement (between 1-6 days) of Fn concentration in culture supernatants incubated with PHA, Fn and associated doses of Fn and PHA, pointed out a significantly increased Fn concentration, on the fourth day, in the cultures incubated with Fn and PHA. On the fourth day of PHA stimulated cultures were found, the increased Fn concentration, as well as the maximum blast transformation.

Plasma fibronectin level in patients with connective tissue diseases.

The level of plasma fibronectin (Fn) was studied in 40 patients with connective tissue diseases. Fn concentration was found increased in patients with rheumatoid arthritis (mean +/- SE, 560 +/- 30 micrograms/ml, p less than 0.01) decreased in patients with mixed connective tissue disease (337 +/- 12 micrograms/ml, p less than 0.

Serial analysis of fibronectin concentration in sera from septic patients.

Serum fibronectin (Fn) level was serially determined in nineteen septic patients. Fn concentration was found significantly decreased in septic patients (mean +/- SE, 120 +/- 11 micrograms/ml) as compared to the control (205 +/- 11 micrograms/ml). The decrease of Fn was correlated with the severity of sepsis; it was persistent and more important in severe septic cases.