Publications by authors named "R Takino"
Physicochemical and biological impacts of light stress on adeno-associated virus serotype 6.
Mol Ther Methods Clin Dev
December 2024
Article Synopsis
- Recombinant adeno-associated virus (rAAV) is being studied as a gene therapy vector, but its response to light exposure has not been fully understood.
- Research on rAAV6 with EGFP showed that light stress resulted in a 20% loss of virus particles and a 90% reduction in biological activity, primarily due to DNA degradation.
- Analysis revealed that light exposure causes specific types of protein and DNA damage, indicating the importance of careful handling and storage of rAAV to preserve its therapeutic effectiveness.
Evolution of the genetic code; Evidence from serine codon use disparity in .
Proc Natl Acad Sci U S A
November 2020
Among the 20 amino acids, three of them-leucine (Leu), arginine (Arg), and serine (Ser)-are encoded by six different codons. In comparison, all of the other 17 amino acids are encoded by either 4, 3, 2, or 1 codon. Peculiarly, Ser is separated into two disparate Ser codon boxes, differing by at least two-base substitutions, in contrast to Leu and Arg, of which codons are mutually exchangeable by a single-base substitution.
View Article and Find Full Text PDFEukaryotic structural maintenance of chromosome proteins (SMC) are major components of cohesin and condensins that regulate chromosome structure and dynamics during cell cycle. We here determine the crystal structure of human condensin SMC hinge heterodimer with ~30 residues of coiled coils. The structure, in conjunction with the hydrogen exchange mass spectrometry analyses, revealed the structural basis for the specific heterodimer formation of eukaryotic SMC and that the coiled coils from two different hinges protrude in the same direction, providing a unique binding surface conducive for binding to single-stranded DNA.
View Article and Find Full Text PDFMagnetoencephalography (MEG) is a record of the magnetic fields produced by the electrical activities of the brain using MEG systems. There are three types of sensors for MEG systems: magnetometer and two types of gradiometer. Among them, two types of gradiometer, axial and planar, have been used worldwide.
View Article and Find Full Text PDFObjective: Transcutaneous electrical nerve stimulation (TENS) affects excitability of the central motor system as well as the somatosensory system. To determine whether TENS has influence on excitability in the sensorimotor cortices of TENS-treated finger muscle, we investigated magnetoencephalogram associated with voluntary, self-paced finger movement before and after TENS.
Methods: High-frequency TENS was applied on the extensor digitorum muscle for 15 min.