Publications by authors named "R Takacs"
Hypoxia-inducible factor-1 (HIF-1) is a heterodimer transcription factor composed of an alpha and a beta subunit. HIF-1α is a master regulator of cellular response to hypoxia by activating the transcription of genes that facilitate metabolic adaptation to hypoxia. Since chondrocytes in mature articular cartilage reside in a hypoxic environment, HIF-1α plays an important role in chondrogenesis and in the physiological lifecycle of articular cartilage.
View Article and Find Full Text PDFEmbryonic limb bud-derived micromass cultures are valuable tools for investigating cartilage development, tissue engineering, and therapeutic strategies for cartilage-related disorders. This collection of fine-tuned protocols used in our laboratories outlines step-by-step procedures for the isolation, expansion, and differentiation of primary mouse limb bud cells into chondrogenic micromass cultures. Key aspects covered in these protocols include synchronized fertilization of mice (Basic Protocol 1), tissue dissection, cell isolation, micromass formation, and culture optimization parameters, such as cell density and medium composition (Basic Protocol 2).
View Article and Find Full Text PDFArticle Synopsis
- * To improve cartilage regeneration, advanced tissue engineering techniques can create a supportive microenvironment, which depends not only on cells and scaffolds but also on biomechanical factors.
- * Understanding how biomechanical signals affect the circadian rhythms of chondrocytes (cartilage cells) is crucial for enhancing cartilage tissue engineering and developing effective treatments for cartilage-related issues.
Article Synopsis
- Much of the skeletal system develops through endochondral ossification, particularly in early fetal life, making it difficult to study initial chondrogenesis in living organisms.
- Researchers are highly interested in improving methods for culturing chondrogenic cells to effectively regenerate articular cartilage and restore joint function.
- The article outlines various protocols, including a micromass culture system for chick embryo-derived cells, high-efficiency cell transfection, and histochemical detection techniques for assessing cartilage matrix production.