Publications by authors named "R T Smolenski"
Article Synopsis
- 5-Fluorouracil (5-FU) is a primary treatment for colorectal cancer and is often combined with leucovorin (LV) to enhance its effectiveness by targeting thymidylate synthase (TS).
- The study compared Arfolitixorin (Arfo), an active form of folate, to LV in combination with 5-FU in patients with colorectal cancer liver metastases to see which produces better TS inhibition and folate levels.
- Results showed that Arfo led to significantly higher levels of folate (MeTHF) and increased TS inhibition compared to LV, indicating that Arfo may be a more effective option, though the difference in TS inhibition wasn't statistically significant.
Research indicates a role for EBI2 receptor in remyelination, demonstrating that its deficiency or antagonism inhibits this process. However, activation of EBI2 with its endogenous ligand, oxysterol 7α,25-dihydroxycholesterol (7α,25OHC), does not enhance remyelination beyond the levels observed in spontaneously remyelinating tissue. We hypothesized that the short half-life of the natural ligand might explain this lack of beneficial effects and tested a synthetic analogue, CF-7α,25OHC, in the cuprizone model.
View Article and Find Full Text PDFThe water-soluble vitamin, vitamin B, also known as cobalamin, plays a crucial role in cellular metabolism, particularly in DNA synthesis, methylation, and mitochondrial functionality. Its deficiency can lead to hematological and neurological disorders; however, the manifestation of these clinical outcomes is relatively late. It leads to difficulties in the early diagnosis of vitamin B deficiency.
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- - Lesch-Nyhan Disease (LND) is a rare genetic disorder caused by mutations in a specific gene, resulting in neurological issues like abnormal movement and self-harm, which are hard to treat.
- - A systematic review of 34 studies found that many had high bias, and there were significant differences in how treatments were applied and evaluated, leading to inconsistent results.
- - The most researched treatments, such as S-Adenosylmethionine and Deep Brain Stimulation, need more investigation, and there is a pressing need for better study designs to improve the assessment of treatment effectiveness in LND.
Nucleotide depletion in hypoxia experimental models of mouse myocardial slices.
Nucleosides Nucleotides Nucleic Acids
November 2024
Objectives: Experimental models to test the effective protection against cardiac ischemia injury are still challenging in pre-clinical studies. The use of myocardial slices creates a special link between testing isolated cardiomyocytes and whole-heart research. In this work, we investigated the effects of oxygen deprivation in a hypoxic chamber and treatment with cobalt chloride (CoCl) on the nucleotide profile in isolated mouse myocardial slices.
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