Publications by authors named "R T Riddle"
Mitochondrial fatty acid oxidation is facilitated by the combined activities of carnitine palmitoyltransferase 1 (Cpt1) and Cpt2, which generate and utilize acylcarnitines, respectively. We compare the response of mice with liver-specific deficiencies in the liver-enriched Cpt1a or the ubiquitously expressed Cpt2 and discover that they display unique metabolic, physiological, and molecular phenotypes. The loss of Cpt1a or Cpt2 results in the induction of the muscle-enriched isoenzyme Cpt1b in hepatocytes in a Pparα-dependent manner.
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- Etomoxir is a medicine that has been used for a long time to block a process called fatty acid β-oxidation in cells.
- Researchers found that etomoxir not only works on one specific protein called Cpt1 but also attaches to many other proteins involved in processing fatty acids in different parts of the cell.
- When they removed a protein called Pex5 that helps transport certain proteins into a part of the cell called the peroxisome, they discovered that etomoxir affected even more proteins, showing that it isn't a precise tool for studying fatty acid oxidation.
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) constitute an appealing tool for drug discovery, disease modeling, and cardiotoxicity screening. However, their physiological immaturity, resembling CMs in the late fetal stage, limits their utility. Herein, we have developed a novel, scalable cell culture medium designed to enhance the maturation of hPSC-CMs.
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- Growth deficiency is a common feature in both Kabuki syndrome 1 (KS1) and Kabuki syndrome 2 (KS2), which are genetic disorders affecting epigenetic regulation.
- A mouse model for KS2, specifically Kdm6atm1d/+, showed reduced bone length and structural abnormalities compared to control mice, along with shorter growth plates due to changes in chondrocyte size.
- RNA sequencing of chondrogenic cell lines from Kdm6a-/- and Kmt2d-/- mice revealed similar gene expression patterns, suggesting that both syndromes share similarities not only in physical characteristics but also at the molecular level.
This study looked at using modified camelina oil to develop sustainable coatings that could replace those derived from petroleum-based materials for use in packaging and other industrial sectors. Solvent-free synthesis of maleic anhydride grafted camelina oil (MCO) was carried out at two different temperatures (200 and 230 °C) to obtain sustainable hydrophobic coating materials for paper substrates. Maleic anhydride grafting of camelina oil was confirmed with attenuated total reflectance-Fourier transform infrared and NMR spectroscopic techniques, and up to 16% grafting of maleic anhydride was achieved, as determined by the titration method.
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