The ion channel TRPM7 regulates zinc-depletion-induced MDMX degradation.

Zinc deficiency has been linked to human diseases, including cancer. MDMX, a crucial zinc-containing negative regulator of p53, has been found to be amplified or overexpressed in various cancers and implicated in the cancer initiation and progression. We report here that zinc depletion by the ion chelator TPEN or Chelex resin results in MDMX protein degradation in a ubiquitination-independent and 20S proteasome-dependent manner.

Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.

Tyramide signal amplification (TSA) is an enzyme-mediated method to enhance the immunohistochemical detection of protein, nucleic acid, or other molecules in situ.Here we describe immunofluorescent detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification compatible double-color immunostaining protocol using primary antibodies from the same host species.

Topical antibiotic treatment for uncomplicated skin and skin structure infections: review of the literature.

Uncomplicated skin infections account for almost 200 million physician-office visits in the USA annually. Treating these infections is estimated to cost in excess of US$350 million each year. The primary etiology of these infections is Staphylococcus aureus, over 60% of which is estimated to be methicillin resistant across the USA.

Modeling the dynamics and control of Schistosoma japonicum transmission on Bohol island, the Philippines.

We have investigated a mathematical model for the transmission of Schistosoma japonicum in the infested region of northeastern Bohol island in the Philippines. The development of transmission models is important for planning control strategies. Since S.

Tensile and tear strength of carrageenan film from Philippine eucheuma species.

The tensile and tear strength of carrageenan film from Philippines Eucheuma species were investigated using NEC tensilon universal-testing machine according to American Society for Testing Materials methods. These properties are important for assessing carrageenan film as packaging material. The kappa and iota types were used in the study.