Publications by authors named "R T LaLonde"
Background: The ability to generate endogenous Cre recombinase drivers using CRISPR-Cas9 knock-in technology allows lineage tracing, cell type specific gene studies, and validation of inferred developmental trajectories from phenotypic and gene expression analyses. This report describes endogenous zebrafish Cre and CreERT2 drivers generated with GeneWeld CRISPR-Cas9 precision targeted integration.
Results: and knock-ins crossed with ubiquitous -based Switch reporters led to broad labeling in expected mesodermal and neural crest-derived lineages in cardiac, pectoral fins, pharyngeal arch, liver, intestine, and mesothelial tissues, as well as enteric neurons.
Capsule networks promise significant benefits over convolutional networks by storing stronger internal representations, and routing information based on the agreement between intermediate representations' projections. Despite this, their success has been limited to small-scale classification datasets due to their computationally expensive nature. Though memory efficient, convolutional capsules impose geometric constraints that fundamentally limit the ability of capsules to model the pose/deformation of objects.
View Article and Find Full Text PDFArticle Synopsis
- The separation of the skull from the pectoral girdle allowed for the evolution of a functional neck in vertebrates, leading to significant changes in their musculoskeletal system.
- Research shows that neck muscles in tetrapods originated from muscle groups in fish, maintaining similar mesodermal sources during embryonic development.
- The adaptation of these existing muscle groups was crucial for the transition from aquatic to terrestrial life, enabling complex head movements in land animals.
Article Synopsis
- The study highlights the importance of the pectoral/shoulder girdle's evolution in the transition from water to land in vertebrates, specifically focusing on the embryonic origins of these structures.
- Researchers identified that the zebrafish pectoral girdle development involves four different embryonic populations and pinpointed the cleithrum as a crucial element lost in current tetrapods.
- The findings suggest that the loss of the cleithrum, essential for neck evolution, is linked to changes in developmental environments, offering insights into how these structures evolved from aquatic to terrestrial forms.
Decades of research have demonstrated that a variety of cognitive biases can affect our judgment and ability to make rational decisions in personal and professional environments. The lengthy, risky, and costly nature of pharmaceutical research and development (R&D) makes it vulnerable to biased decision-making. Moreover, cognitive biases can play a role in regulatory and clinical decision-making, the latter impacting diagnostic and treatment decisions in the therapeutic use of medicines.
View Article and Find Full Text PDF