Publications by authors named "R T Kroemer"
The age-associated deterioration in cellular and organismal functions associates with dysregulation of nutrient-sensing pathways and disabled autophagy. The reactivation of autophagic flux may prevent or ameliorate age-related metabolic dysfunctions. Non-toxic compounds endowed with the capacity to reduce the overall levels of protein acetylation and to induce autophagy have been categorized as caloric restriction mimetics (CRMs).
View Article and Find Full Text PDFEntropy is an important energetic quantity determining the progression of chemical processes. We propose a new approach to obtain hydration entropy directly from probability density functions in state space. We demonstrate the validity of our approach for a series of cations in aqueous solution.
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- Researchers found that inhibiting STAT3 significantly boosts autophagy, both in lab settings and in living organisms.
- Overexpression of different STAT3 variants hampers autophagy during starvation, showing that STAT3 plays a crucial role in regulating this process.
- The study reveals that STAT3 interacts with PKR, and inhibiting STAT3 disrupts this interaction, promoting autophagy through PKR activation and eIF2α phosphorylation.
Article Synopsis
- The study introduces a new computer-based protocol for predicting how mutations affect protein binding by simulating conformational changes and calculating free energy variations.
- The protocol was tested on 173 mutations across 7 protein complexes, revealing that combining two prediction methods led to identifying mutations that enhanced binding, with a success rate of 45%.
- For more complex mutations requiring multiple base changes, the success rate rose to 63%, and the protocol successfully detected 89% of significant mutation hotspots in 56 alanine scanning tests.
To address the question of whether established or experimental anticancer chemotherapeutics can exert their cytotoxic effects by autophagy, we performed a high-content screen on a set of cytotoxic agents. We simultaneously determined parameters of autophagy, apoptosis and necrosis on cells exposed to -1400 compounds. Many agents induced a 'pure' autophagic, apoptotic or necrotic phenotype, whereas less than 100 simultaneously induced autophagy, apoptosis and necrosis.
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