Long-term control of Legionella species in potable water after a nosocomial legionellosis outbreak in an intensive care unit.

Environmental and patient-care control measures were initiated in response to an outbreak of legionellosis in an intensive care unit in 1992. The measures included maintaining elevated potable hot-water temperatures following superheating and using sterile water for administrations through nasogastric tubes. Legionella species remained below detectable levels in the potable hot-water system upon reevaluation in 1999.

A one-step RT-PCR assay using an enzyme-linked detection system for the diagnosis of enterovirus meningitis.

Background: Enteroviruses are the most common cause of meningitis in the United States, with an estimated 50000-75000 cases each year. Enteroviral meningitis (EVM) is frequently a diagnosis of exclusion, as viral cultures lack sensitivity and require prolonged incubation periods.

Objective: To develop a sensitive and rapid test for the diagnosis of EVM.