Publications by authors named "R Siret"

Data on the esters of hydroxycinnamic acids (HCAs) from the cell walls of wine grapes (Cabernet franc) and cider apples (Douce Moen and Guillevic) were acquired. Caffeic acid, -coumaric acid (CA) and ferulic acid (FA) monomers were identified by HPLC-UV/MS. Means to limit the oxidative degradation during cell wall preparation were assessed by the yield of HCA recovered after alkaline extraction.

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Phenolic compounds in fruit are involved in responses to biotic and abiotic stresses and are responsible for organoleptic properties. To establish the distribution of these secondary metabolites at the tissue and sub-cellular scales, mapping of fluorescence in apple epidermis and outer cortex tissue in cryogenic condition was performed after deep-UV excitation at 275 nm. Douce Moën and Guillevic cider apple varieties were sampled and frozen after harvest, after 30 days at 4 °C and after 20 days at room temperature.

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Article Synopsis
  • The study addresses challenges in observing small diffusible components in water-rich plant tissues, like ions and phenolic compounds.
  • A new experimental method combining infusion staining, cryo-fixation, and fluorescence microscopy was successfully applied to analyze the structure of apple and grape tissues for localizing important compounds.
  • This cryo-technique proves effective and offers potential for future imaging of various diffusible elements in hydrated plant tissues.
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Background: The evolution of the sensory and instrumental properties of grape seeds was investigated during berry development, with a focus on the effects of the harvest season and growing location.

Results: The sensory and instrumental texture analyses gave a consistent description of the ripening process. Moreover, the effect of maturation on the seed sensory descriptors was clearly influenced by the harvest season, and astringency was the most appropriate sensory attribute for the assessment of grape seed ripening.

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Using 20 SSR markers well scattered across the 19 grape chromosomes, we analyzed 4,370 accessions of the INRA grape repository at Vassal, mostly cultivars of Vitis vinifera subsp. sativa (3,727), but also accessions of V. vinifera subsp.

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