The integration of PCR testing in serum into the diagnostic algorithm of suspected acute Q fever in an endemic setting.

Unlabelled: Serum polymerase chain reaction (PCR) for the detection of DNA has been suggested for rapid Q fever diagnosis. We evaluated the role of PCR testing in serum in the diagnosis of acute Q fever in an endemic setting. We examined patients suspected of acute Q fever tested for -specific serum real-time PCR in a tertiary hospital between January 2019 toand December 2022.

NGS in the clinical microbiology settings.

We hypothesized that targeted NGS sequencing might have an advantage over Sanger sequencing, especially in polymicrobial infections. The study included 55 specimens from 51 patients. We compared targeted NGS to Sanger sequencing in clinical samples submitted for Sanger sequencing.

Aging of human epidermis: reversal of aging changes correlates with reversal of keratinocyte fas expression and apoptosis.

The goal of this study was to determine the role of Fas-mediated apoptosis in human epidermal aging. Epidermal Fas expression and apoptosis are increased in aged human skin. Aging changes of human epidermis, including decreased epidermal thickness and proliferation, are reversed following grafting of human skin to SCID (severe combined immunodeficiency) mice.

Ageing of human epidermis: the role of apoptosis, Fas and telomerase.

Background: Aged human epidermis is characterized by morphological changes including flattening of the dermal-epidermal junction and a decrease in thickness.

Objectives: To determine the roles of proliferation, apoptosis, Fas (CD95), Fas ligand (FasL) and telomerase in changes of human epidermis during ageing.

Methods: Human epidermis from aged subjects (n = 14; mean age 70.