[Antibiotic resistance plasmids in various strains of Staphylococcus aureus].

Plasmids with the molecular weights of 1.6 to 21.0 MD were detected in Staphylococcus aureus.

[Distribution and transcription of determinants homologous to the alpha-hemolysin (hlY) gene in the genomes of HLY- and HLY+ staphylococci].

The molecular organization of the alpha-hemolysin gene from Staphylococcus aureus strain O15 has been studied. Hybridization of the DNA from this strain with the [32P]-labeled cloned fragment containing alpha-hemolysin gene has shown the studied gene to be unique in the genome of the strain. Sequences homologous to the gene were not found to be dispersed along the genome of Staphylococcus aureus O15.

[Cloning of alpha-hemolysin gene from Staphylococcus aureus strain 015].

The genomic library of Staphylococcus aureus O15 has been constructed on the EMBL-3 vector. The synthetic oligonucleotide probes to N- and C-end regions of alpha-hemolysin permitted identification of the recombinant bacteriophage clone RS-1 containing a gene for this protein. The restriction map of the cloned fragment has been constructed for restriction endonucleases SalGI, EcoRV, PstI, PvuII.