[Effect of halogenated quaternary ammonium on cultured tumor cells].

This work reports the action of one halogenic quaternary ammonium compound on the in-vitro proliferation of different lines of human cancer cells. IC 50 inhibition growth was observed at a concentration of 2.10(-6) mol and T1 growth at 3.

[Effect of chlorine-methylated quaternary ammonium on human gastric cancer cell proliferation].

This work reports the action of some choline derivatives on the proliferation of the human gastric cancer cell line HGT-1 which does not secrete mucus or carcino-embryonic antigen, but express histamine H2 and somatostatin receptors. The structural family of the tested molecules (GMS-003F, GMS-005F et GMS-010F) belongs to the chloruro-methylated quaternary ammonium. A dose-dependent growth inhibitory effect, significant but weak (20%), was observed at 1-10 mM concentrations of GMS-003F, GMS-005F and choline.

Interest of simultaneous ultrastructural characterization of morphology, cytochemistry and immune phenotype in a case of putative hybrid acute leukaemia.

A case of acute leukaemia is reported in which blast cells expressed some B-related antigens (namely the CALLA antigen) and no peroxidase activity at the optical level; however, some mature granular cells contained Auer rods. Simultaneous characterization of ultrastructural morphology, cytochemistry and immune phenotype was performed. There was an apparent mutual exclusion in the expression of myeloperoxidase activity and the CALLA antigen, and a heterogeneity in the CALLA expression among the blastic population.

Acute myeloid leukemia with giant inclusions: cytochemical and ultrastructural study.

Giant granules formation was investigated in myeloblasts of a patient with acute myelogenous leukemia by means of the combined techniques of peroxidase cytochemistry both in light and electron microscopy. Several pathologic features were noted: first an abnormal packaging of peroxidase in the peripheral area in large azurophilic granulations, second the progressive enlargement of huge vacuolar inclusions resulting from the interaction and fusion of large azurophilic granules with each other, with normal-sized primary granules and with cytoplasmic components. Microcrystalline structure could not be found in giant vacuoles no in vacuolar inclusions resembling Auer bodies.

Plasma transferable inhibition of BCG induced subcutaneous inflammation in human cancer.

When a small dacron sponge disc containing live BCG was implanted subcutaneously in healthy subjects it became infiltrated with leukocytes which at 24 hours mainly involved neutrophils. this mycobacterial-induced inflammatory response was markedly impaired in patients with cancer. This inhibitory effect could be reproduced by injecting the plasma of these patients into guinea-pigs bearing identical discs.