Lymphocytic Na(+)-H+ exchange increases after an oral glucose challenge.

The effects of oral glucose challenge on plasma glucose concentration, plasma insulin concentration, arterial blood pressure, cytosolic pH (pHi), cytosolic free Na+ concentration ([Na+]i), and cellular Na(+)-H+ exchange activity were investigated in 16 healthy subjects. The pHi, [Na+]i, and Na(+)-H+ exchange activity were measured in intact lymphocytes by using the fluorescent dye technique. The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na(+)-H+ exchange activity, measured as change of pHi per second (control [0 hours], 5.

Filling state of intracellular Ca2+ pools triggers trans plasma membrane Na+ and Ca2+ influx by a tyrosine kinase-dependent pathway.

The relations between the filling state of intracellular calcium stores that are regulated by the endoplasmic Ca(2+)-ATPase and trans plasma membrane sodium and calcium influx were investigated. The effects of specific inhibition of endoplasmic Ca(2+)-ATPase by thapsigargin, cyclopiazonic acid, and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (BHQ) on cytosolic free sodium concentration ([Na+]i) and cytosolic free calcium concentration ([Ca2+]i) were evaluated in lymphocytes from healthy subjects using the fluorescent dyes sodium-binding benzofuran isophthalate and fura2. The specific inhibition of endoplasmic Ca(2+)-ATPase by thapsigargin, cyclopiazonic acid, or BHQ increased lymphocytic [Na+]i and [Ca2+]i.

Effect of magnesium on cytosolic free sodium in human lymphocytes.

Cytosolic free sodium concentration ([Na+]i) was measured in intact human lymphocytes using the novel sodium-sensitive fluorescent dye sodium-binding benzofuran-isophthalate. In the presence of 1 mmol/l external Mg2+ the resting [Na+]i was significantly lower compared to the value in the absence of external Mg2+ (22.7 +/- 1.

Gas formation after renal artery embolisation: genesis and clinical relevance.

Preoperative embolisation of renal carcinomas has several pros and cons for the patient. The negative aspects can be summarized as 'post-infarction' syndrome'. Radiologically, intrarenal gas formation is always evident.