Acute Slice Patch-Clamp Recordings to Characterize Neural Populations in the Hippocampal Subgranular Zone.

Whole-cell patch clamp allows the evaluation of neuronal excitability and characterization of synaptic transmission. With this technique, it is possible to characterize the neuron maturation level and its integration into the hippocampal circuit. This facilitates the identification of the different stages of neural progenitor cells in the adult brain and their contribution to hippocampal function.

DNA lesions can frequently precede DNA:RNA hybrid accumulation.

While DNA:RNA hybrids contribute to multiple genomic transactions, their unscheduled formation is a recognized source of DNA lesions. Here, through a suite of systematic screens, we rather observed that a wide range of yeast mutant situations primarily triggering DNA damage actually leads to hybrid accumulation. Focusing on Okazaki fragment processing, we establish that genic hybrids can actually form as a consequence of replication-born discontinuities such as unprocessed flaps or unligated Okazaki fragments.

Guided Selective Preservation of Tooth (SPOT) Protocol: Fully Guided Socket-Shield Technique.

The socket-shield procedure focuses on soft- and hard-tissue preservation of the extraction socket in an immediate implant procedure. Selective preservation of tooth (SPOT) is a tooth-guided protocol for socket-shield preparation for both anterior and posterior teeth. It allows for consistent apex removal as well as shield preparation, implant site preparation, and immediate implant placement.

Canopy functional trait variation across Earth's tropical forests.

Tropical forest canopies are the biosphere's most concentrated atmospheric interface for carbon, water and energy. However, in most Earth System Models, the diverse and heterogeneous tropical forest biome is represented as a largely uniform ecosystem with either a singular or a small number of fixed canopy ecophysiological properties. This situation arises, in part, from a lack of understanding about how and why the functional properties of tropical forest canopies vary geographically.

Caregivers' Experiences of Sleep Systems for Children with Complex Neurodisability: A Qualitative Study.

Aim: To understand caregivers' experiences of implementing sleep systems for children with complex neurodisability. This knowledge would help develop and refine the evidence base to inform clinical practice to improve care and support increased engagement and adherence to this approach.

Methods: Semi-structured interviews were conducted with nine caregivers (7 mothers, 2 fathers) of eight children aged 4-17 years (mean age 7.