Say it ain't : a microbiology nudge comment leads to less treatment of respiratory colonization.

Objective: To describe the effect of a (SM) respiratory culture nudge on antibiotic use in colonized patients.

Design: IRB-approved quasi-experiment.

Setting: Five acute-care hospitals in Michigan.

Alternative splicing modulates chromatin interactome and phase separation of the RIF1 C-terminal domain.

RIF1 (RAP1 interacting factor) fulfills diverse roles in DNA double-strand break repair, DNA replication, and nuclear organization. RIF1 is expressed as two splice variants, RIF1-Long (RIF1-L) and RIF1-Short (RIF1-S), from the alternative splicing (AS) of Exon 32 (Ex32) which encodes a 26 aa Ser/Lys-rich cassette peptide in the RIF1 C-terminal domain (CTD). Here we demonstrate that Ex32 inclusion was repressed by DNA damage and oncogenesis but peaked at G/M phase of the cell cycle.

Use of whole genomic sequencing to detect New Delhi metallo-B-lactamase (NDM)-producing outbreak associated with endoscopic procedures.

Background: Whole-genome sequencing (WGS) has emerged as an alternative genotyping tool for outbreak investigations in the healthcare setting. We describe the investigation and control of a New Delhi metallo-B-lactamase (NDM)-producing cluster in Southeast Michigan.

Methods: Michigan Bureau of Laboratories identified several closely related NDM-producing isolates with WGS.

Leveraging stewardship to promote ceftriaxone use in severe infections with low- and no-risk AmpC Enterobacterales.

AmpC β-lactamases are associated with development of ceftriaxone resistance despite initial susceptibility, but the risk of AmpC derepression is not equal among Enterobacterales. The purpose of this study was to evaluate the impact of an AmpC stewardship intervention on the definitive treatment of low- and no-risk Enterobacterales. This was an IRB-approved, single pre-test, post-test quasi-experiment at a 5-hospital system.