Publications by authors named "R S Prins"
Article Synopsis
- Glioblastoma is described as immunologically "cold," making it resistant to solo immune-checkpoint inhibitors (ICI) like pembrolizumab, although neoadjuvant use may improve survival based on prior studies.
- A study involving 25 additional patients analyzed tumor tissue for gene signatures and found that neoadjuvant pembrolizumab led to decreased cancer proliferation genes and increased T-cell activity, indicating a specific response to this treatment.
- Despite observing these molecular changes, the study did not confirm an overall survival benefit from neoadjuvant pembrolizumab, suggesting that some patients may inherently resist ICI and may need additional therapies for effective treatment.
Article Synopsis
- HPV16, a type of Human Papillomavirus, requires a specific endocytic process involving actin to infect epithelial cells, although the exact mechanisms of this process are not fully understood.
- This study identifies WAVE1 and WAVE2 proteins as key players in actin polymerization that facilitates HPV16's entry into cells, demonstrating their importance through gene silencing and genome editing techniques.
- Observations showed that HPV16, WAVE1, WAVE2, and actin localize together on the cell surface, and HPV16 activates the formation of filopodia—extensions of the cell membrane—during the viral entry process, underscoring the role of actin dynamics in viral infection.
Oligo pools are array-synthesized, user-defined mixtures of single-stranded oligonucleotides that can be used as a source of synthetic DNA for library cloning. While currently offering the most affordable source of synthetic DNA, oligo pools also come with limitations such as a maximum synthesis length (approximately 350 bases), a higher error rate compared to alternative synthesis methods, and the presence of truncated molecules in the pool due to incomplete synthesis. Here, we provide users with a comprehensive protocol that details how oligo pools can be used in combination with Golden Gate cloning to create user-defined protein mutant libraries, as well as single-guide RNA libraries for CRISPR applications.
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