Enhancing chemical signal transformation in lateral flow assays using aptamer-architectured plasmonic nanozymes and para-phenylenediamine.

The widespread adoption and commercialization of lateral flow assays (LFAs) for clinical diagnosis have been hindered by limitations in sensitivity, specificity, and the absence of quantitative data. To address these challenges, we developed aptamer-architectured gold nanoparticles as nanozymes that catalytically convert -phenylenediamine (PPD) into Bandrowski's base (BB), thereby amplifying signal strength and sensitivity. The physiochemical properties of the nanozymes were characterized and their specific binding efficiency was demonstrated using experimental studies.

Ultrasensitive Detection of Blood-Based Alzheimer's Disease Biomarkers: A Comprehensive SERS-Immunoassay Platform Enhanced by Machine Learning.

Accurate and early disease detection is crucial for improving patient care, but traditional diagnostic methods often fail to identify diseases in their early stages, leading to delayed treatment outcomes. Early diagnosis using blood derivatives as a source for biomarkers is particularly important for managing Alzheimer's disease (AD). This study introduces a novel approach for the precise and ultrasensitive detection of multiple core AD biomarkers (Aβ, Aβ, p-tau, and t-tau) using surface-enhanced Raman spectroscopy (SERS) combined with machine-learning algorithms.

Atomically Precise Fluorescent Gold Nanocluster as a Barrier-Permeable and Brain-Specific Imaging Probe.

Photonic nanomaterials play a crucial role in facilitating the necessary signal for optical brain imaging, presenting a promising avenue for early diagnosis of brain-related disorders. However, the blood-brain barrier (BBB) presents a significant challenge, blocking the entry of most molecules or materials from the bloodstream into the brain. To overcome this, photonic nanocrystals in the form of gold clusters (LAuC) with size less than 3 nm, have been developed, with Levodopa conjugated to LAuC (Dop@LAuC) for targeted brain imaging.

Nitrogen-doped carbon dots: a novel biosensing platform for selective norfloxacin detection and bioimaging.

Incomplete metabolism and non-biodegradable nature of norfloxacin (NORx) lead to its persistent residues in the environment and food, potentially fostering the emergence of antibiotic resistance and posing a significant threat to public health. Hence, we developed a norfloxacin sensor employing hydrothermally synthesized N-doped carbon dots (N-Ch-CQDs) from chitosan and PEI demonstrated high sensitivity and specificity towards the antibiotic detection. The quantum yield of excitation-dependent emission of N-Ch-CQDs was effectively tuned from 4.

Porphyrin and doxorubicin mediated nanoarchitectonics of copper clusters: a bimodal theranostics for cancer diagnosis and treatment .

Nanoarchitectonics, an emerging strategy, presents a promising alternative for developing highly efficient next-generation functional materials. Multifunctional materials developed using nanoarchitectonics help to mimic biological molecules. Porphyrin-based molecules can be effectively utilized to design such assemblies.