Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping.

The Notch receptor is a pleiotropic signaling protein that translates intercellular ligand interactions into changes in gene expression via the nuclear localization of the Notch intracellular Domain (NICD). Using a combination of immunohistochemistry, RNA in situ, Optogenetics and super-resolution live imaging of transcription in human cells, we show that the N1ICD can form condensates that positively facilitate Notch target gene expression. We determined that N1ICD undergoes Phase Separation Coupled Percolation (PSCP) into transcriptional condensates, which recruit, enrich, and encapsulate a broad set of core transcriptional proteins.

Is Culture Learned? The Neglected Role of Evoking Events.

Although no one disputes that the transmission of culture depends on social learning, a capacity that has enabled humans, unlike other animals, to modify cultural practices across generations, this review argues that cultural change can also be evoked by environmental events leading to an alteration in the configuration of an habitual behavioural repertoire. An evoked mechanism allows latent or normally suppressed behaviour to emerge. Cannibalism and warfare are put forward as examples.

Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping.

The Notch receptor is a pleiotropic signaling protein that translates intercellular ligand interactions into changes in gene expression the nuclear localization of the Notch intracellular Domain (NICD). Using a combination of immunohistochemistry, RNA , Optogenetics and super-resolution live imaging of transcription in human cells, we show that the N1ICD can form condensates that positively facilitate Notch target gene expression. We determined that N1ICD undergoes Phase Separation Coupled Percolation (PSCP) into transcriptional condensates, which recruit, enrich, and encapsulate a broad set of core transcriptional proteins.

Chronic Disease Risk of Family Child Care Professionals:: Results of a Statewide Survey of Health and Wellbeing Indicators.

Objective: To document the chronic disease risk factors and prevalence rate of family child care professionals. Given that a significant number of young children spend time in family child care (FCC) settings, these environments are an important focus for efforts to improve children's health.

Methods: Data were collected in fall 2021 from a statewide survey of licensed FCC professionals in one mid-Atlantic state (N=541), using validated questionnaires to assess health status, including chronic diseases like high blood pressure, diabetes, and asthma, as well as nutrition and physical activity.