Multivariate analysis of prognostic features in malignant pleural effusions from breast cancer patients.

To identify prognostic indicators in breast cancer patients with malignant pleural effusions, we analyzed the cytopathologic features of 57 fluids representing the first pathologic diagnosis of a distant metastasis in these women. The specimens were analyzed prior to reviewing the clinical records. The median survival of 55 patients who died of the disease was five months following the effusion diagnosis (range, 1-114).

Immunostaining of small cytologic specimens. Facilitation with cell transfer.

Immunocytochemical study of cytologic specimens is often limited by the number of slides containing diagnostic cells. This study examined the effectiveness of transferring cells from a single smear to multiple slides in order to perform a battery of immunocytochemical stains on limited material. Immunostaining performed on four effusions and five fine needle aspirates yielded the expected results for most of the antibodies commonly employed in diagnostic pathology.

Bloodless splenic surgery: the safe warm-ischemic time.

To assess the feasibility of a technique of bloodless splenic surgery, experiments were performed to determine the safe warm-ischemic time of the spleen. Ten mongrel dogs were divided into two groups. Group I (n = 5) underwent division of all collateral splenic vessels, followed by total splenic artery and vein occlusion for 3 hours.

p53 expression in precancerous gastric lesions: an immunohistochemical study of PAb 1801 monoclonal antibody on adenomatous and hyperplastic gastric polyps.

To determine the chronology of p53 mutation in the gastric carcinogenic sequence, we studied p53 overexpression in premalignant lesions, including 17 adenomatous polyps (10/17 surrounded by intestinal metaplasia and 11/17 harboring foci of adenocarcinoma or severe dysplasia), and 18 hyperplastic polyps (4/18 with focal adenomatous changes). Immunohistochemistry with PAb 1801 monoclonal antibody was performed on archival material; p53 nuclear staining was seen in 10/17 adenomas, but was limited to the foci of adenocarcinoma in three cases. Five adenomas with foci of severe dysplasia or carcinoma were nonreactive.

Blood group antigens in Barrett's esophagus and associated adenocarcinomas.

A panel of monoclonal antibodies (MoAbs) against BGAg A, B, and H and Lewis a, b, X, and Y (Le a, Le b, X, Y) was used to characterize and evaluate the blood group antigens (BGAg) immunohistochemistry in Barrett's esophagus (BE) and associated adenocarcinomas (ACA). Thirteen cases of ACA arising in metaplastic columnar epithelium of the distal esophagus were reviewed (12 men; 1 woman; mean age: 59). Twelve cases had juxtaposed BE of different types with low-grade dysplasia in five and high-grade dysplasia in three.