Publications by authors named "R Raychowdhury"
Article Synopsis
- - We studied 1,130 E3 ligases and their roles in the inflammatory response of primary dendritic cells using Perturb-seq, revealing their significant impact on different types of dendritic cells and macrophages.
- - E3 ligases and their adaptors work together but interact with different substrate recognition adaptors, influencing various processes in dendritic cell development and function.
- - A deep learning model named comβVAE was developed to predict outcomes of new E3 ligase combinations, showing that the E3 regulatory network is linked to genetic variations and abnormal gene expression in immune-related human diseases.
Macrophages use an array of innate immune sensors to detect intracellular pathogens and to tailor effective antimicrobial responses. In addition, extrinsic activation with the cytokine IFN-γ is often required as well to tip the scales of the host-pathogen balance toward pathogen restriction. However, little is known about how host-pathogen sensing impacts the antimicrobial IFN-γ-activated state.
View Article and Find Full Text PDFIn this study, we report that the TLR4 ligand, LPS, and TLR3 ligand polyinosinic:polycytidylic acid failed to activate IRF3 or STAT1 in bone marrow-derived macrophages (BMMs) isolated from two independently generated lines of Rosa26-integrated Cas9-expressing C57BL/6J (B6) mice. RNA-sequencing analysis reveals that hundreds to thousands of genes including IFN-stimulated genes were differentially expressed in BMMs from these Cas9 strains compared with B6 upon LPS stimulation. Furthermore, the NF-κB signaling axis and TRIF-mediated necroptosis were also strongly reduced in response to LPS and polyinosinic:polycytidylic acid.
View Article and Find Full Text PDFWhile hundreds of genes are induced by type I interferons, their roles in restricting the influenza virus life cycle remain mostly unknown. Using a loss-of-function CRISPR screen in cells prestimulated with interferon beta (IFN-β), we identified a small number of factors required for restricting influenza A virus replication. In addition to known components of the interferon signaling pathway, we found that replication termination factor 2 (RTF2) restricts influenza virus at the nuclear stage (and perhaps other stages) of the viral life cycle, based on several lines of evidence.
View Article and Find Full Text PDFIntroduction: Heavy metals are a major environmental threat in India and there are several health risks associated with it. The aim of this study was to investigate the relationship between the blood levels of lead, cadmium, arsenic, and mercury and a sensoneurial hearing loss in children aged one to ten years.
Method: Heavy metal blood levels were determined using inductively coupled plasma mass spectrometry, with appropriate quality control.