Publications by authors named "R Radi"
The active site Cys residue in glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is sensitive to oxidation by hydrogen peroxide (H2O2), with this resulting in enzyme inactivation. This re-routes the carbon flux from glycolysis to the pentose phosphate pathway favoring the formation of NADPH and synthetic intermediates required for antioxidant defense and repair systems. Consequently, GAPDH inactivation serves as a redox switch for metabolic adaptation under conditions of oxidative stress.
View Article and Find Full Text PDFCantú syndrome is a multisystem disorder caused by gain-of-function (GOF) mutations in KCNJ8 and ABCC9, the genes encoding the pore-forming inward rectifier Kir6.1 and regulatory sulfonylurea receptor SUR2B subunits, respectively, of vascular ATP-sensitive K+ (KATP) channels. In this study, we investigated changes in the vascular endothelium in mice in which Cantú syndrome-associated Kcnj8 or Abcc9 mutations were knocked in to the endogenous loci.
View Article and Find Full Text PDFArticle Synopsis
- - The choice of energy substrate, like glucose or galactose, significantly affects the metabolic pathways of cells in culture, particularly in endothelial cells which have low mitochondrial oxidation.
- - Endothelial cells grown in galactose medium exhibit higher mitochondrial oxidative capacity, a more interconnected mitochondrial network, and increased cell-to-cell communication compared to those grown in glucose medium.
- - Galactose culture conditions lead to elevated levels of the redox regulator FOXO3 and reduced levels of Nrf2 in bovine aortic endothelial cells, suggesting that galactose media is better for studying mitochondrial-related processes and redox signaling.
Peroxymonocarbonate (HCO/HOOCO) is produced by the reversible reaction of CO/HCO with HO ( = 0.33 M, pH 7.0).
View Article and Find Full Text PDFNicotine e-cigarettes are a safe and effective way to help patients stop smoking.
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