Publications by authors named "R Rabelo"
Assessment of shock index in healthy cats and in cats presenting to an emergency room with shock.
J Vet Emerg Crit Care (San Antonio)
January 2025
Objective: To prospectively compare the shock index (SI) in a population of healthy cats with a population of cats presenting to the emergency room (ER) deemed to be in a state of shock.
Design: Prospective cohort study of cats.
Setting: University teaching hospital.
Background: The main challenge in new drug development is accurately predicting the human response in preclinical models.
Methods: In this study, we developed three different intestinal barrier models using advanced biofabrication techniques: (i) a manual model containing Caco-2 and HT-29 cells on a collagen bed, (ii) a manual model with a Caco-2/HT-29 layer on a HDFn-laden collagen layer, and (iii) a 3D bioprinted model incorporating both cellular layers. Each model was rigorously tested for its ability to simulate a functional intestinal membrane.
Upon exposure to biological environments, nanoparticles are rapidly coated with biomolecules, predominantly proteins, which alter their colloidal stability, biodistribution, and cell interactions. Despite extensive efforts to investigate the nanoparticles' fate, only a few studies use high-resolution characterization methods that allow in-depth characterization, and the existing methodologies are unable to differentiate particles internalized at the onset of incubation from those taken up toward the end of an incubation period. In this study, these limitations related to incubation disparities are overcame and precisely monitored the spatiotemporal displacement of colloidally stable protein corona-coated nanoparticles within cells.
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- - Elephant grass (Cenchrus purpureus) is an efficient CO2 sequester and a promising source for biodiesel and cattle feed, but it has recently shown signs of a disease causing necrotic leaf spots in Brazil's Tocantins state, affecting both young and mature leaves significantly during the rainy season.
- - Infected leaves were collected and processed in a lab, revealing fungal colonies that exhibited distinct morphological characteristics, such as greyish coloration and curved conidia, indicating a specific type of fungal infection.
- - DNA analysis identified the fungal isolate as Curvularia chiangmaiensis, with a high genetic similarity to known reference sequences, suggesting a potential link to the observed disease impacting elephant grass in the region.*
Importance: Microplastic (MP) pollution is an emerging environmental and health concern. While MPs have been detected in various human tissues, their presence in the human brain has not been documented, raising important questions about potential neurotoxic effects and the mechanisms by which MPs might reach brain tissues.
Objective: To determine the presence of MPs in the human olfactory bulb and to analyze their characteristics such as size, morphology, color, and polymeric composition.