Species identification of ruminant milk by genotyping of the κ-casein gene.

The development of molecular genetic and bioinformatic systems for identifying the species of milk and the raw material composition of dairy products is of great scientific and practical importance with the purpose of introducing developments in the system for controlling the turnover of falsified products. The aim of the research is to develop a method of PCR-RFLP analysis for species identification of milk and dairy products from agricultural ruminant animals by the κ-casein gene (CSN3) with the possibility of qualitative and relative quantitative assessment of species-specific DNA of the tested biomaterial. The objects of research were samples of raw milk and milk powder, pasteurized cream, and hard and semi-hard cheeses.

Methodological Approaches to DNA Authentication of Foods, Wines and Raw Materials for Their Production.

DNA authentication of wines is a process of verifying their authenticity by genetic identification of the main plant component. The sample preparation of experimental and commercial wines was carried out by precipitation of wine debris by centrifugation with preliminary exposure with precipitators and co-precipitators, including developed macro- and micro-volume methods applicable to white or red wines, using polyvinylpyrrolidone as a co-precipitator. Addition of 2-mercaptoethanol and proteinase K to the lysing solution made it possible to adapt the technology for DNA extraction from the precipitated wine debris.

Effects of critical fluctuations of storage temperature on the quality of dry dairy product.

Whole milk powder (WMP) is a universal raw material component that can overcome the problem of seasonality of raw milk. It can be used to provide high-nutritional products to remote areas experiencing a raw milk shortage. Its long shelf life depends on the conditions of storage and transportation, which are recommended to be carried out in a range from 0 to 10°C.

[MOLECULAR-GENETIC ANALYSIS OF MICROORGANISMS WITH INTRAEPITHELIAL INVASION ISOLATED FROM PATIENTS WITH COLORECTAL CANCER].

The facultative aerobic bacteria isolated from the mucosa of rectum in patients with colorectal cancer in the zone of malignant tumor and neighboring normal mucosa was studied using molecular-genetic methods. The species attribution of bacteria was implemented using the cultural-morphological analysis and sequencing of the 16S rRNA locus. The microorganisms with the intraepithelial invasion to rectal mucosa isolated were identified as representatives of the adherent-invasive (AIEC) subgroup of Escherichia coli and species Klebsiella pneumonia.

[Genotypic identification of the bovine leukemia virus].

Phylogenetic analysis of the sequenced segments of the provirus BLV locus env-gene and the strategy of the PCR-PDRF-genotyping consistent with phylogenetic classification of pathogenic agent and suggested in our works provided taxonomic identification of BLV isolates identified in cattle in Tatarstan (Russian Federation) as representatives of the 4th, 7th, and 8th BLV genotypes. Of 100 identified isolates, 64 represent the 4th BLV genotype, 28 representatives of BLV belong to cluster of the 7th genotype, whereas the other 8 samples of the provirus belong to the new 8th genotype of pathologic agent. The strategy VBL PCR-PDRF-genotyping suggested in our work on the basis of 5 restriction endonucleases (PvuII, SspI, HphI, HaeIII, and BstYI) provided correct genotyping identification of the viral pathogen.