Publications by authors named "R R Cutler"
Unlabelled: As the principal lipid transporter in the human brain, apolipoprotein E (ApoE) is tasked with the transport and protection of highly vulnerable lipids required to support and remodel neuronal membranes, in a process that is dependent on ApoE receptors. Human allele variants that encode proteins differing only in the number of cysteine (Cys)-to-arginine (Arg) exchanges (ApoE2 [2 Cys], ApoE3 [1 Cys], ApoE4 [0 Cys]) comprise the strongest genetic risk factor for sporadic Alzheimer's disease (AD); however, the molecular feature(s) and resultant mechanisms that underlie these isoform-dependent effects are unknown. One signature feature of Cys is the capacity to form disulfide (Cys-Cys) bridges, which are required to form disulfide bridge-linked dimers and multimers.
View Article and Find Full Text PDFSemantic memory, a repository for concepts and factual information, plays a vital role in acquiring and retrieving knowledge. This study explores the impact of age-related knowledge accumulation on semantic cognition, investigating whether a denser representational space affects retrieval processes. Using a semantic feature verification task, we employ both behavioral (reaction time; RT) and neurophysiological (event-related potential; ERP) measures to explore these dynamics across young and older adults.
View Article and Find Full Text PDFThus far, multiple techniques for single cell analysis have been developed, yet we lack a relatively simple tool to assess DNA and RNA from the same cell at whole-transcriptome and whole-genome depths. Here we present an updated method for physical separation of cytoplasmic RNA from the nuclei, which allows for simultaneous studies of DNA and RNA from the same single cell. The method consists of three steps-(1) immobilization of a single cell on solid substrate, (2) hypotonic lysis of immobilized single cell, and (3) separation of cytosol containing aqueous phase and immobilized nucleus.
View Article and Find Full Text PDFArticle Synopsis
- Scientists created a new method called schPTM to study tiny details of proteins in individual cells, especially how they change after certain treatments.
- This method can identify different protein changes (68 types) and can tell the difference between cells that were treated and those that weren't.
- It helps researchers learn more about how cells respond differently to treatments and understand the complex signals, or "codes," in the proteins.
Time-Restricted Feeding Reduces Atherosclerosis in LDLR KO Mice but Not in ApoE Knockout Mice.
Arterioscler Thromb Vasc Biol
September 2024
Article Synopsis
- Dyslipidemia, a risk factor for cardiovascular disease, can be mitigated by time-restricted feeding (TRF), which limits food intake to a 12-hour window, resulting in reduced weight gain and cholesterol levels in preclinical mouse models.
- In studies involving mice with LDLR mutations, TRF significantly decreased hypercholesterolemia and atherosclerosis by promoting lipid metabolism and excretion, demonstrating potential benefits for heart health.
- The findings suggest that TRF could serve as an effective lifestyle intervention for reducing cardiovascular risks, particularly in individuals with LDLR-related conditions, though it may not be effective for those lacking the ApoE protein.