Publications by authors named "R Pottie"
Article Synopsis
- Alternative splicing is crucial for plant gene regulation and is facilitated by the spliceosome, which processes pre-mRNA.
- A suppressor screen identified a mutation in the SME1 protein that reduced cell death in catalase-deficient plants, likely due to inhibited splicing.
- Both genetic and chemical disruptions to spliceosome function increased stress responses in mutants, suggesting that altering spliceosome components enhances plants' resilience to oxidative stress.
Gene regulation is a dynamic process in which transcription factors (TFs) play an important role in controlling spatiotemporal gene expression. To enhance our global understanding of regulatory interactions in Arabidopsis thaliana, different regulatory input networks capturing complementary information about DNA motifs, open chromatin, TF-binding and expression-based regulatory interactions were combined using a supervised learning approach, resulting in an integrated gene regulatory network (iGRN) covering 1,491 TFs and 31,393 target genes (1.7 million interactions).
View Article and Find Full Text PDFSignaling events triggered by hydrogen peroxide (H2O2) regulate plant growth and defense by orchestrating a genome-wide transcriptional reprogramming. However, the specific mechanisms that govern H2O2-dependent gene expression are still poorly understood. Here, we identify the Arabidopsis Mediator complex subunit MED8 as a regulator of H2O2 responses.
View Article and Find Full Text PDFPhysical damage to cells leads to the release of immunomodulatory peptides to elicit a wound defense response in the surrounding tissue. In , the plant elicitor peptide 1 (Pep1) is processed from its protein precursor, PRECURSOR OF PEP1 (PROPEP1). We demonstrate that upon damage, both at the tissue and single-cell levels, the cysteine protease METACASPASE4 (MC4) is instantly and spatiotemporally activated by binding high levels of Ca and is necessary and sufficient for Pep1 maturation.
View Article and Find Full Text PDFObjective: To compare the speed and quality of induction of general anaesthesia using three different inhalant agents and one intravenous agent, in healthy dogs undergoing desexing surgery.
Materials And Methods: Less excitable dogs were not premedicated; others were premedicated with intramuscular acepromazine and morphine. Anaesthesia induction protocol was randomly assigned, with halothane, isoflurane or sevoflurane delivered by mask, or propofol delivered intravenously.