Preparation and Mechano-Functional Characterization of PEGylated Fibrin Hydrogels: Impact of Thrombin Concentration.

Three-dimensional (3D) neuronal cultures grown in hydrogels are promising platforms to design brain-like neuronal networks in vitro. However, the optimal properties of such cultures must be tuned to ensure a hydrogel matrix sufficiently porous to promote healthy development but also sufficiently rigid for structural support. Such an optimization is difficult since it implies the exploration of different hydrogel compositions and, at the same time, a functional analysis to validate neuronal culture viability.

Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels.

Three-dimensional (3D) neuronal cultures are valuable models for studying brain complexity in vitro, and the choice of the bulk material in which the neurons grow is a crucial factor in establishing successful cultures. Indeed, neuronal development and network functionality are influenced by the mechanical properties of the selected material; in turn, these properties may change due to neuron-matrix interactions that alter the microstructure of the material. To advance our understanding of the interplay between neurons and their environment, here we utilized a PEGylated fibrin hydrogel as a scaffold for mouse primary neuronal cultures and carried out a rheological characterization of the scaffold over a three-week period, both with and without cells.

Continuously Sheared Granular Matter Reproduces in Detail Seismicity Laws.

We introduce a shear experiment that quantitatively reproduces the main laws of seismicity. By continuously and slowly shearing a compressed monolayer of disks in a ringlike geometry, our system delivers events of frictional failures with energies following a Gutenberg-Richter law. Moreover, foreshocks and aftershocks are described by Omori laws and interevent times also follow exactly the same distribution as real earthquakes, showing the existence of memory of past events.

Spatiotemporal Organization of Correlated Local Activity within Global Avalanches in Slowly Driven Interfaces.

We study the jerky response of slowly driven fronts in disordered media, just above the depinning transition. We focus on how spatially disconnected clusters of internally correlated activity lead to large-scale velocity fluctuations in the form of global avalanches and identify three different ways in which local activity clusters may organize within a global avalanche, depending on the distance to criticality. Our analysis provides new scaling relations between the power-law exponents of the statistical distributions of sizes and durations of local bursts and global avalanches.

Revealing the structure of a granular medium through ballistic sound propagation.

We study the propagation of sound through a bidimensional granular medium consisting of photoelastic disks, which are packed into different crystalline and disordered structures. Acoustic sensors placed at the boundaries of the system capture the acoustic signal produced by a local and well-controlled mechanical excitation. By compressing the system, we find that the speed of the ballistic part of the acoustic wave behaves as a power law of the applied force with both exponent and prefactor sensitive to the internal geometry of the contact network.