[Perioperative immunologic changes in colorectal cancer patients].

The aim of the study was to investigate the perioperative immunological profile in colon cancer patients and possible correlations with disease. To investigate the changes in immune mediators profile induced by tumor resection, we assessed the serum levels of cytokines (IL-6, IL-8, IL-10, IFN-gamma, TNF-alpha), chemokines (MIP-1alpha, MCP-1, ENA-78) and growth factors (VEGF, bFGF) in colon cancer patients before, during and after surgery and compared the results with those measured for a group of healthy controls. We have used XMap profiling technology (Luminex) that allows simultaneous measurement of multiple parameters in small volumes of samples.

Influence of tumor cell culture supernatants on macrophage functional polarization: in vitro models of macrophage-tumor environment interaction.

Aims And Background: Macrophages are heterogeneous cells with extensive functional plasticity; they can change their functional profiles repeatedly in response to environmental changes anywhere between their extreme phenotypical programs (labeled as M1 and M2 polarization, respectively). In terms of antitumoral immune response, M1 macrophages are considered to be beneficial, while M2 macrophages supposedly promote tumor progression. Tumor-associated macrophages (TAMs) represent a major leukocyte population present in many tumors.

Bacterial extract cantastim activates macrophages via TLR-2.

CANTASTIM is a second generation bacterial immunomodulator. The aim of this study was to examine the mechanism by which bacterial immunomodulator CANTASTIM induces production of inflammatory cytokines in monocytes/macrophages. Proinflammatory cytokines were induced in PMA-differentiated THP-1 cells by stimulation with TLR agonists and CANTASTIM in the presence or absence of anti-TLR blocking antibodies or isotype matched control antibodies.

Serum perioperative profile of cytokines in patients with squamous cell carcinoma of the larynx.

Objective: Altered immune, inflammatory, and angiogenesis responses have been noticed in head and neck cancer, and many of these responses have been associated with a poor clinical outcome. The objective of this study was to evaluate several immune mediators in the sera of patients with squamous cell carcinoma (SCC) of the larynx undergoing curative surgery in connection with clinicopathologic factors.

Methods: Multiplex analysis of cytokines (interleukin [IL]-6, IL-8, IL-10, tumour necrosis factor α [TNF-α], interferon-γ [IFN-γ]), chemokines (monocyte chemoattractant protein 1 [MCP-1], macrophage inflammatory protein 1α [MIP-1α], and epithelial neutrophil-activating protein 78 [ENA-78]), and growth factors (vascular endothelial growth factor and basic fibroblast growth factor) in the serum of patients with laryngeal cancer and healthy controls was performed using xMap technology.

Serum levels of adipokines resistin and leptin in patients with colon cancer.

Adipose tissue displays characteristics of an endocrine organ releasing a number of adipocyte-specific factors known as adipocytokines. It has been recently suggested that adipocytokines may play a role in pathogenesis and progression of certain cancers, in particular in colorectal cancer. The aim of this study was to investigate the association between several blood adipocytokine levels and clinicopathological characteristics of colon cancer patients undergoing surgery.