Correlation of prostate cancer nuclear deoxyribonucleic acid, size, shape and Gleason grade with pathological stage at radical prostatectomy.

Using image cytometry and a video planimetry unit, various deoxyribonucleic acid (DNA) measurements, nuclear size and shape factors, and Gleason grade were correlated with capsular penetration, seminal vesicle invasion and lymph node involvement in 113 radical prostatectomy specimens. Percentage of nondiploid cells was the best DNA measurement and standard deviation of nuclear area was the best size measurement correlating with capsular penetration. However, stepwise regression analysis demonstrated that Gleason grade was the only independent predictor of capsular penetration.

Use of nuclear morphometry, gleason histologic scoring, clinical stage, and age to predict disease-free survival among patients with prostate cancer.

Background: Currently, there are no accurate methods for predicting metastases or time to disease progression for patients with clinically localized prostate cancer after surgery.

Methods: In this report, histologic sections were studied from prostate cancer specimens from 100 men with clinically localized prostate cancer (clinical Stages A1 [9 cases], A2 [24 cases], B1 [27 cases], and B2 [40 cases]; pathologic Stages A1 [9 cases], A2 [22 cases], B [23 cases], C1 [8 cases], and D1 [38 cases]) to determine whether nuclear morphometry--when analyzed with clinical stage, pathologic parameters, and age in a multivariate fashion--would predict time to disease progression.

Results: These patients were treated with surgery alone for their clinically localized disease and were observed after surgery until disease progression or death.

Nuclear morphology of prostatic carcinoma: comparison of computerized image analysis (CAS 200) versus video planimetry (DynaCELL).

We compared mean nuclear size and mean nuclear shape (nuclear roundness) on Feulgen-stained smears from 113 cases of prostatic carcinoma analyzed by DynaCELL system at x 100 magnification versus CAS system at x 40 where DNA can be assessed simultaneously. Correlation coefficients were 0.67 for size and -0.

A comparison of nuclear morphometry and Gleason grade as a predictor of prognosis in stage A2 prostate cancer: a critical analysis.

The natural history of stage A2 prostate cancer is unknown. Previous studies from this institution have shown that, without treatment, a third of the men with clinically localized stage A2 prostatic adenocarcinoma will have disease progression within 4 years. Presently, most patients who present with stage A2 prostate cancer receive surgical or radiation therapy.