Electron-stimulated reactions in layered CO/H2O films: hydrogen atom diffusion and the sequential hydrogenation of CO to methanol.

Low-energy (100 eV) electron-stimulated reactions in layered H2O/CO/H2O ices are investigated. For CO layers buried in amorphous solid water (ASW) films at depths of 50 monolayers (ML) or less from the vacuum interface, both oxidation and reduction reactions are observed. However, for CO buried more deeply in ASW films, only the reduction of CO to methanol is observed.

Pruritic erythematous maculopapular rash.

A trip to the beach left this patient with an unusual rash where his bathing suit had been.

Canine coronavirus in Australian dogs.

Objective: To estimate the frequency of serum antibodies (IgG and IgM) to canine coronavirus (CCV) in the Australian dog population and evaluate the role of CCV as a causative agent of gastroenteritis.

Design: A serological survey of antibodies to CCV among different dog populations.

Procedure: The development and characterisation of an indirect ELISA for the detection of antibodies (IgG and IgM) to CCV was undertaken.

Identification of canine coronavirus strains from feces by S gene nested PCR and molecular characterization of a new Australian isolate.

A nested PCR (nPCR) assay for the detection of canine coronavirus (CCV) in fecal samples is described. The target sequence for the assay was a 514-bp fragment within the spike (S) glycoprotein gene. The sensitivity of the assay is extremely high, detecting as little as 25 50% tissue culture infective doses per g of unprocessed feces.

Replication of Australian porcine isolates of Ileal symbiont intracellularis in tissue culture.

Ileal symbiont intracellularis (ISI) isolated from Australian cases of PIA and PHE was replicated in the rat ileum enterocyte cell line IEC 18. The number of ISI within cells varied, as did the number of ISI infected within the monolayer. At 24 h post infection a large number of cells were infected with approximately 100 ISI per cell.