Neddylation drives myofibrillogenesis in the developing heart.

Neddylation is a highly conserved post-translational modification that plays critical roles in various cellular processes through the modulation of cullins and non-cullin substrates. While neddylation is known to be essential for embryonic development, tumor growth, and organogenesis of different tissues, its role in cardiogenesis remains unexplored. Here, we investigated the role of neddylation in early cardiac development by deleting the gene encoding a regulatory subunit of the NEDD8-specific E1 activating enzyme, Nae1, globally and in a heart-specific fashion via Nkx2-5.

An electronic phase-space Hamiltonian approach for electronic current density and vibrational circular dichroism.

The Born-Oppenheimer framework stipulates that chemistry and physics occur on potential energy surfaces VBO(X) parameterized by a nuclear coordinate X, which are built by diagonalizing a BO Hamiltonian ĤBO(X). However, such a framework cannot recover many measurable chemical and physical features, including vibrational circular dichroism spectra. In this article, we show that a phase-space electronic Hamiltonian ĤPS(X,P), parameterized by both nuclear position X and momentum P, with a similar computational cost as solving ĤBO(X), can recover not just experimental vibrational circular dichroism signals but also a meaningful electronic current density that explains the features of the vibrational circular dichroism rotational strengths.

Inhibition of cardiomyocyte neddylation impairs embryonic cardiac morphogenesis.

Heart development is a complex spatiotemporal process involving a series of orchestrated morphogenic events that result in the formation of an efficient pumping organ. How posttranslational mechanisms regulate heart development remains poorly understood. Therefore, we investigate how neddylation, the attachment of NEDD8 to target proteins, coordinates cardiogenesis.

Comparing the Diagnostic Yield of Germline Exome Versus Panel Sequencing in the Diverse Population of the Texas KidsCanSeq Pediatric Cancer Study.

Purpose: To evaluate the relative diagnostic yield of clinical germline genomic tests in a diverse pediatric cancer population.

Patients And Methods: The KidsCanSeq study enrolled pediatric cancer patients across six sites in Texas. Germline analysis included both exome sequencing and a therapy-focused pediatric cancer gene panel.

A Phase-Space Electronic Hamiltonian For Vibrational Circular Dichroism.

We show empirically that a phase-space non-Born-Oppenheimer electronic Hamiltonian approach to quantum chemistry (where the electronic Hamiltonian is parametrized by both nuclear position and momentum, (,)) is both a practical and accurate means to recover vibrational circular dichroism spectra. We further hypothesize that such a phase-space approach may lead to very new dynamical physics beyond spectroscopic circular dichroism, with potential implications for understanding chiral induced spin selectivity (CISS), noting that classical phase-space approaches conserve the total nuclear plus electronic momentum, whereas classical Born-Oppenheimer approaches do not (they conserve only the nuclear momentum).