In silico and experimental characterization of a new polyextremophilic subtilisin-like protease from and its application as a laundry detergent additive.

Considering the current growing interest in new and improved enzymes for use in a variety of applications, the present study aimed to characterize a novel detergent-stable serine alkaline protease from the extremophilic actinobacterium TL13 (MmSP) using a combined in silico and experimental approach. The MmSP showed a close phylogenetic relationship with high molecular weight S8 peptidases of species. Moreover, its physical and chemical parameters computed using Expasy's ProtParam tool revealed that MmSP is hydrophilic, halophilic and thermo-alkali stable.

Exogenous application of spermidine mitigates the adverse effects of drought stress in faba bean ( L.).

In Tunisia, drought stress is a major environmental factor limiting crop production and causing relatively low and unstable faba bean yields. In the present study, we explored the putative role of spermidine (0.5, 1, 1.

Roots of the xerophyte host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria.

Bacterial communities associated with roots of , exposed to arid conditions, were investigated with a combination of cultural and metataxonomic approaches. Traditional culture-based techniques were used and 32 isolates from the irradiated roots were identified as belonging to Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria phyla. Four actinobacterial strains were shown to be ionizing-radiation (IR)-resistant: sp.

Morphological influence of BiVO nanostructures on peroxymonosulfate activation for highly efficient catalytic degradation of rhodamine B.

Bismuth vanadate (BiVO) nanostructured films were prepared and successfully applied for peroxymonosulfate (PMS) activation for the degradation of rhodamine B (RhB) in aqueous solution. The BiVO thin films were obtained by thermal reaction between electrodeposited bismuth (Bi) films and vanadium precursor. The as-prepared BiVO porous, nanoflowers, and cluster nanostructures were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy, and BET analysis.

Physiological responses and expression of sugar associated genes in faba bean ( L.) exposed to osmotic stress.

Unlabelled: Faba bean ( L.) is the major food legume crop in Tunisia. However, its growth and yield is strongly affected by water-limited environments.