Publications by authors named "R Ossendorff"
Inflammation models with the proinflammatory cytokine interleukin-1β (IL-1β) are widely used in the in vitro investigation of new therapeutic approaches for osteoarthritis (OA). The aim of this study was to systematically analyze the influence of IL-1β in a 3D chondral pellet culture model. Bovine articular chondrocytes were cultured to passage 3 and then placed in pellet culture.
View Article and Find Full Text PDFBackground: Symptomatic cartilage defects of the knee joint are frequently diagnosed and can be treated with different available surgical methods. Nevertheless, there is currently no gold standard treatment for all indications. Minced cartilage implantation is increasingly coming into focus as a refined surgical technique.
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- The case discusses a rare instance of bilateral traumatic sternoclavicular dislocations in a 27-year-old male cyclist who experienced significant injuries during a race.
- Immediate and collaborative surgical intervention was crucial for the patient's recovery, with the absence of complications noted during a 1-year follow-up.
- The report emphasizes the importance of comprehensive trauma management in complex cases, showcasing this injury's rarity and the effectiveness of quick transportation to specialized care facilities.
Tumor necrosis factor alpha (TNFα), as a key pro-inflammatory cytokine, plays a central role in joint diseases. In recent years, numerous models of TNFα-induced cartilage inflammation have been developed. However, due to the significant differences between these models and the lack of consensus in their construction, it becomes difficult to compare the results of different studies.
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- The study investigates the role of TNFα in inflammatory models for osteoarthritis, focusing on how its effects vary with concentration and chondrocyte growth stages.
- Bovine chondrocytes were used, cultured in a 3D pellet, and exposed to TNFα for 48 hours, revealing that a concentration of 10 ng/mL was required to significantly induce inflammation.
- Results showed that higher passages of chondrocytes (especially passage 3) were more sensitive to TNFα, affecting both gene expression of catabolic/inflammatory markers and protein levels of certain cytokines.