Immune analysis of -genetically modified potato by in-silico analysis and animal mode.

The safety of feeding transgenic potato (), resistant to the potato tuber moth, to Wistar rats was examined from an immunological perspective. The genetically modified potato (GMP) was harbouring and genes as target and selectable marker genes, respectively. In-silico analysis reconfirmed that Cry1Ab and NPTII protein sequences have no significant homology to known toxins or known allergens.

The effect of increasing feeding frequency on performance, plasma hormones and metabolites, and hepatic lipid metabolism of broiler breeder hens.

An experiment was conducted to study the effects of feeding regimens on reproductive performance, plasma hormone and metabolite levels, and hepatic lipid metabolism of Cobb 500 broiler breeder hens from 26 to 38 wk of age. Seventy-two birds were used in a completely randomized design with 3 treatments, each replicated 4 times. Treatments were as follows: 1) once a day feeding, in which birds were fed once a day at 0615 h (control), 2) twice a day feeding, in which daily allocated feed was fed in 2 equal meals at 0615 and 1215 h, and 3) thrice a day feeding in which daily allocated feed was offered in 3 equal meals at 0615, 1215, and 1815 h.

Power of microsatellite markers for analysis of genetic variation and parentage verification in sheep.

We report the genetic analysis of 100 individuals of an elite breeding flock of Afshari sheep with a selected set of eighteen microsatellite markers. A full characterization of this set of eighteen loci was carried out generating allele frequency distributions that were used to estimate the genetic information content of these loci, including genetic variability, inbreeding, individual and parent verification parameters. Disregarding MCMA26 monomorphic pattern, microsatellite loci showed moderate level of polymorphism, as such totally 102 alleles were detected with a mean number of 6 alleles per locus.