Effects of dipyridamole on prostaglandin synthesis in human fibroblasts.

The effect of varied doses of dipyridamole (Persantin) on the arachidonic acid metabolites prostaglandin (PG)I2, PGE2 and thromboxane (TX)A2 has been studied in human skin fibroblasts cultured in vitro. A 24-h exposure to the drug induced a dose-dependent increase of all the examined prostaglandins (PG's). Bradykinin stimulation of cells that had been preexposed to the drug induced a continuous increase in PG-production as compared to controls, despite the absence of the drug.

Human erythropoiesis in vitro and the source of burst-promoting activity in a serum-free system.

Bovine serum albumin (BSA) can markedly increase the number of size of erythropoietic bursts produced by mononuclear cells from human bone marrow and peripheral blood, and reduce the threshold amount of erythropoietin (Epo) required for initial burst formation. The purpose of this study was to determine a possible burst-promoting activity (BPA) of BSA. The experiments were performed in a miniaturized agar system, in which the addition of sheep Epo to cultures with or without BSA was delayed for five days.

CFU-gm assay, cytochemical and electron microscopic studies in agar in patients with preleukemic syndrome and aplastic anemia.

Thirty-seven patients with chronic cytopenia were studied using a CFU-gm assay in agar. Cell proliferation was evaluated on days 2, 3, 5, 7, and 10 of incubation. Growth patterns were different in cultures of hematologically healthy persons versus patients with preleukemic syndrome (PL) and aplastic anemia (AA).