Evaluation of the potential of multi-trait PGPR isolates as inoculants for maize () growth.

Background And Objectives: Plant growth-promoting rhizobacteria (PGPR) with a diverse set of traits can improve crop yield in agriculture. The current study aimed to evaluate the potential of multi-trait PGPR isolates as inoculants for maize growth.

Materials And Methods: In this study, 23 bacterial isolates were initially screened from maize plant rhizosphere.

A biomimetic sperm selection device for routine sperm selection.

Research Question: Can a biomimetic microfluidic sperm sorter isolate motile sperm while minimizing DNA damage in comparison with density gradient centrifugation (DGC)?

Design: This was a two-phase study of 61 men, consisting of a proof-of-concept study with 21 donated semen samples in a university research laboratory, followed by a diagnostic andrology study with 40 consenting patients who presented at a fertility clinic for semen diagnostics. Each sample was split to perform DGC and microfluidic sperm selection (one-step sperm selection with 15 min of incubation) side-by-side. Outcomes evaluated included concentration, progressive motility, and DNA fragmentation index (DFI) of raw semen, and sperm isolated using DGC and the microfluidic device.

Repeated pulses of ultrasound maintain sperm motility.

Sperm motility is a primary criterion for selecting viable and functional sperm in assisted reproduction, where the most motile sperm are used to increase the likelihood of successful conception. Traditional chemical agents to enhance motility pose embryo-toxicity risks, necessitating safer alternatives. This study investigates the use of low-intensity pulsed ultrasound exposure as a non-invasive treatment within an acoustofluidic device to maintain sperm motility.

A novel CCDC112-dependent process of sperm midpiece formation and epididymal maturation.

The sperm mitochondrial sheath has proposed functions in structural support and energy production for motility. Here we define coiled coil domain containing protein 112, CCDC112, as crucial for male fertility, specifically in the assembly and function of the mitochondrial sheath. We unveiled a previously unrecognised process of epididymal mitochondrial sheath maturation.