Publications by authors named "R Nardini"
The mechanisms of the innate immunity control of equine infectious anemia virus in horses are not yet widely described. Equine monocytes isolated from the peripheral blood of three Equine infectious anemia (EIA) seronegative horses were differentiated in vitro into macrophages that gave rise to mixed cell populations morphologically referable to M1 and M2 phenotypes. The addition of two equine recombinant cytokines and two EIA virus reference strains, Miami and Wyoming, induced a more specific cell differentiation, and as for other species, IFNγ and IL4 stimulation polarized horse macrophages respectively towards the M1 and the M2 phenotypes.
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- Equine hepacivirus (EqHV) is an RNA virus linked to mild hepatitis in horses and is present worldwide, but Italy lacks comprehensive data on its prevalence.
- A study involving 1,801 horse serum samples across various categories and regions in Italy found a national prevalence of 4.27%, with no significant differences among categories or regions.
- The research identified three known EqHV subtypes in Italy and suggested a potential fourth subtype, highlighting the need for EqHV to be included in hepatitis diagnostic protocols and blood product safety measures.
Article Synopsis
- - European brown hare syndrome (EBHS) is a deadly viral disease affecting mainly European brown hares, caused by the EBHS virus (EBHSV), which poses a significant threat to populations such as the vulnerable Italian hare.
- - This study investigated two free-ranging Italian hares found dead in Central Italy, identifying EBHS through harmful lesions, viral detection in their livers, and genetic analysis confirming the presence of EBHSV.
- - The EBHSV strain found in these hares had a close genetic match to a strain from Germany in 2019, highlighting the spread of EBHS and emphasizing the need for conservation efforts for vulnerable species like the Italian hare in protected areas.
Lentiviruses, including equine infectious anemia virus (EIAV), are considered viral quasispecies because of their intrinsic genetic, structural and phenotypic variability. Immunoenzymatic tests (ELISA) for EIAV reported in the literature were obtained mainly by using the capsid protein p26, which is derived almost exclusively from a single strain (Wyoming), and do not reflect the great potential epitopic variability of the EIAV quasispecies. In this investigation, the GenBank database was exploited in a systematic approach to design a set of representative protein antigens useful for EIAV serodiagnosis.
View Article and Find Full Text PDFPopulation growth and industrialization have led to a race for greater food and supply productivity. As a result, the occupation and population of forest areas, contact with wildlife and their respective parasites and vectors, the trafficking and consumption of wildlife, the pollution of water sources, and the accumulation of waste occur more frequently. Concurrently, the agricultural and livestock production for human consumption has accelerated, often in a disorderly way, leading to the deforestation of areas that are essential for the planet's climatic and ecological balance.
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