Publications by authors named "R Namba"
We developed a novel next-generation sequencing-based single-nucleotide polymorphism (SNP) genotyping method for second- and third-degree kinship analysis, and designed 1144- and 2045-SNP panels using one (Set A) and two sets (Sets A and B) of primers. These SNP loci were analyzed in 120 Japanese individuals, and likelihood ratios (LRs) for kinship discrimination were calculated to evaluate the effect of number of SNP loci in simulated analysis. Likelihood evaluation was performed using DNA profiles from two individuals, namely, the unknown and one reference relative.
View Article and Find Full Text PDFBackground: Kounis syndrome (KS) is an underdiagnosed disease. The management of the disease remains elusive because of its infrequency.
Case Presentation: A 78-year-old man with anaphylactic shock was admitted to our hospital 2 h after multiple bee stings.
DNA methylation modifications are known to influence epigenetic phenomena and have been a focus of forensic science research for some time. Degraded DNA after bisulfite treatment is widely used in DNA methylation analysis. In this study, we analyzed methylation levels at 12 CpG sites of four selected genomic regions by pyrosequencing after bisulfite treatment.
View Article and Find Full Text PDFArticle Synopsis
- This study focuses on the development of thermostable locked nucleic acid (LNA) primers to improve age prediction based on DNA methylation analysis, particularly requiring less DNA than traditional methods.
- The researchers tested various amounts of DNA (10, 5, and 2 ng) and found that LNA primers significantly enhanced signal intensity and accuracy, especially for the EDARADD and SST genes.
- Results indicated that using LNA primers with just 10 ng of DNA led to a low mean error of 3.88 years in age prediction, showcasing their effectiveness in methylation analysis with minimal DNA input.
Introduction: Centers of excellence and bundled payment models have driven perioperative optimization and surgical site infection (SSI) prevention with decolonization protocols and antibiotic prophylaxis strategies. We sought to evaluate time trends in the incidence of deep SSI and its causative organisms after six orthopaedic procedures in a US-based integrated healthcare system.
Methods: We conducted a population-level time-trend study using data from Kaiser Permanente's orthopaedic registries.