Publications by authors named "R N Wilton"
Wastewater testing can inform public health action as a component of polio outbreak response. During 2022-2023, a total of 7 US jurisdictions (5 states and 2 cities) participated in prospective or retrospective testing of wastewater for poliovirus after a paralytic polio case was identified in New York state. Two distinct vaccine-derived poliovirus type 2 viruses were detected in wastewater from New York state and New York City during 2022, representing 2 separate importation events.
View Article and Find Full Text PDFPseudomonas putida KT2440 is a robust, aromatic catabolic bacterium that has been widely engineered to convert bio-based and waste-based feedstocks to target products. Towards industrial domestication of P. putida KT2440, rational genome reduction has been previously conducted, resulting in P.
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- Bisulfite sequencing is essential for studying genomic methylation, crucial for understanding various health conditions like cancer, but processing the vast amounts of data generated is computationally intensive and time-consuming.
- * BiocMAP is a new workflow for methylation analysis that includes two modules: one for fast read alignment using GPU technology and another for data processing that can run even without a GPU, making it more accessible for researchers.
- * The pipeline is designed for efficient memory use and is compatible with R's Bioconductor, ensuring it can handle large WGBS datasets while promoting reproducibility across different computing setups.
Motivation: Read alignment is an essential first step in the characterization of DNA sequence variation. The accuracy of variant-calling results depends not only on the quality of read alignment and variant-calling software but also on the interaction between these complex software tools.
Results: In this review, we evaluate short-read aligner performance with the goal of optimizing germline variant-calling accuracy.
Multispectral, multiplex immunofluorescence (mIF) microscopy has been used to great effect in research to identify cellular co-expression profiles and spatial relationships within tissue, providing a myriad of diagnostic advantages. As these technologies mature, it is essential that image data from mIF microscopes is reproducible and standardizable across devices. We sought to characterize and correct differences in illumination intensity and spectral sensitivity between three multispectral microscopes.
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