Formation and characterization of acid-soluble soybean protein with chitosan addition: effects of hydrothermal treatment and homogenization.

Background: The development of acid-soluble soybean protein (ASSP) is a dynamic field with ongoing research aimed at exploring its emulsifying, foaming, and gelling properties. These properties can affect the texture, stability, and sensory attributes of food. Innovations in processing technologies, such as freeze-drying, hydrothermal treatment, and homogenization processing, are being made to enhance the quality of ASSP, which are crucial for its application in food products.

Poly-γ-glutamic acid alleviates slow transit constipation by regulating aquaporin and gut microbes.

Slow transit constipation (STC) is a prevalent gastrointestinal disorder caused by colon dysfunction. Poly-γ-glutamic acid (γ-PGA), an anionic polymer known for its moisture retention, degradability, and food safety, was studied for its effects on loperamide-induced STC in mice. Treatment with γ-PGA for one week significantly increased both defecation frequency and fecal water content, with the high-dose group (10 g/kg/d) restoring fecal water content to 34.

In vivo prime editing rescues photoreceptor degeneration in nonsense mutant retinitis pigmentosa.

The next-generation gene editing tool, prime editing (PE), is adept at correcting point mutations precisely with high editing efficiency and rare off-target events and shows promising therapeutic value in treating hereditary diseases. Retinitis pigmentosa (RP) is the most common type of inherited retinal dystrophy and is characterized by progressive degeneration of retinal photoreceptors and, consequently, visual decline. To date, effective treatments for RP are lacking.

Exploration of cfDNA landscape in NIPT and clinical utilities of cfDNA based gene expression inference in prenatal diagnostics.

Cell-free DNA (cfDNA) is a dynamic biomarker reflecting the physiological state of the body. Its unique physical and biochemical properties, inherited from the tissue of origin, enable a wide range of clinical applications. From methylation patterns and fragmentation profiles to genetic variants, cfDNA holds immense potential for diagnosing and monitoring various diseases, including cancer.

Extracellular vesicles from Lactobacillus fermentum enhance intestinal barrier integrity and restore gut microbial homeostasis in experimental murine colitis.

Background: Lactobacillus fermentum (L. fermentum) has been shown to improve intestinal health and treat colitis; however, its precise efficacy and mechanisms in inflammatory bowel disease (IBD) remain unclear.

Objectives: This study aimed to evaluate whether L.