Publications by authors named "R N DuBois"
Porcine astrovirus 4 (PoAstV4) has been recently associated with respiratory disease in pigs. In order to understand the scope of PoAstV4 infections and to support the development of a vaccine to combat PoAstV4 disease in pigs, we designed and produced a recombinant PoAstV4 capsid spike protein for use as an antigen in serological assays and for potential future use as a vaccine antigen. Structural prediction of the full-length PoAstV4 capsid protein guided the design of the recombinant PoAstV4 capsid spike domain expression plasmid.
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- The study focuses on the antibody response to the Moderna Spikevax COVID-19 vaccine, highlighting the importance of understanding this immune response to pathogens.
- It introduces a new method that combines mass spectrometry and B-cell sequencing to analyze human plasma-derived polyclonal IgG, addressing limitations in traditional B-cell repertoire sequencing.
- The researchers created 12 recombinant antibodies from their sequencing data, with six showing strong binding affinities and neutralizing capabilities against the target antigen.
Article Synopsis
- Short Bowel Syndrome (SBS) is a significant cause of intestinal failure in children, and this study evaluates the long-term outcomes of the surgical technique called serial transverse enteroplasty (STEP) used to treat it.
- The study involved a review of 36 children who underwent STEP between 2000 and 2022, examining their medical histories, surgical details, and nutritional support.
- Results showed an increase in bowel length and a decrease in dependency on parenteral nutrition, with many children experiencing improved digestive symptoms and growth after the procedure, indicating STEP can be a beneficial option for managing SBS in children.
Human astroviruses (HAstVs) are a leading cause of viral childhood diarrhea that infect nearly every individual during their lifetime. Although human astroviruses are highly prevalent, no approved vaccine currently exists. Antibody responses appear to play an important role in protection from HAstV infection, however knowledge about the neutralizing epitope landscape is lacking, as only 3 neutralizing antibody epitopes have previously been determined.
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