Publications by authors named "R N Ben"
Recent advances in the biosynthesis and production optimization of gentamicin: A critical review.
Synth Syst Biotechnol
November 2024
Gentamicin, an aminoglycoside antibiotic, is generated by a few species within the genus and has garnered significant attention due to its broad-spectrum efficacy in combating numerous infectious diseases. Comprising a complex array of closely related aminoglycoside compounds, the gentamicin B and C complexes emerge as particularly pertinent in clinical contexts. This review outlines the latest advancements in the biosynthesis and production of gentamicin, commencing with a comprehensive overview of its biosynthetic pathway.
View Article and Find Full Text PDFThe successful use of human induced pluripotent stem cells (iPSCs) for research or clinical applications requires the development of robust, efficient, and reproducible cryopreservation protocols. After cryopreservation, the survival rate of iPSCs is suboptimal and cell line-dependent. We assessed the use of ice recrystallization inhibitors (IRIs) for cryopreservation of human iPSCs.
View Article and Find Full Text PDFDespite the routine use of cryopreservation for the storage of biological materials, its outcomes are often sub-optimal (including reduced post-thaw viability, recovery, and functionality) due to the damage caused by uncontrolled ice growth. Traditional cryoprotective agents (CPAs), including dimethyl sulfoxide (DMSO), fail to prevent damage caused by ice growth and concerns over CPA cytotoxicity have fostered an increased interest in developing improved CPAs and cryoprotection strategies. The inhibition of ice recrystallization by natural antifreeze (glyco)proteins [AF(G)Ps] to improve cryopreservation outcomes has been examined; however, the ice binding properties of these substances and their challenging large-scale production make them poor CPA candidates.
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- Fructose metabolism is linked to several diseases, but a lack of suitable imaging tools has made studying its role in these conditions difficult.
- Researchers developed a new radiotracer, [F]4-FDF, using a molecular design inspired by aldolase, an enzyme crucial for fructose breakdown, achieving high effectiveness in metabolic imaging.
- The study found that [F]4-FDF has low uptake in healthy brain and heart tissues while successfully mapping inflammatory responses, indicating its potential as a valuable tool for diagnosing fructose metabolism-related diseases.
Article Synopsis
- Human induced pluripotent stem cells (iPSCs) and iPSC-derived neurons (iPSC-Ns) show promise for cell-based therapies in regenerative medicine, but effective cryopreservation is key for their successful use.
- A study found that N-aryl-D-aldonamides (specifically 2FA), an ice recrystallization inhibitor, improved the viability and recovery of iPSCs after thawing without affecting their pluripotency, while also enhancing the functional activity of iPSC-Ns compared to traditional cryopreservation methods.
- Optimizing cryopreservation techniques using IRIs could significantly advance the viability and utility of iPSCs and iPSC-Ns, making them more viable for clinical applications and