Targeted Deletion in the Basal Body Protein Talpid3 Leads to Loss of Primary Cilia in Embryonic Stem Cells and Defective Lineage-Specific Differentiation.

Talpid3 is a basal body protein required for the formation of primary cilia, an organelle involved in signal transduction. Here, we asked if Talpid3 has a role in the regulation of differentiation and/or self-renewal of ES cells and whether cells lacking cilia due to a deletion in Talpid3 can be reprogrammed to induced pluripotent stem (iPS) cells. We show that mouse embryonic limb fibroblasts which lack primary cilia with a targeted deletion in the () gene can be efficiently reprogrammed to iPS cells.

Chlorthalidone vs Hydrochlorothiazide and Kidney Outcomes in Patients With Hypertension: A Secondary Analysis of a Randomized Clinical Trial.

Importance: Hypertension is a risk factor for the development and progression of chronic kidney disease (CKD). It is unclear whether different thiazide diuretics have a differential impact on kidney outcomes.

Objective: To compare kidney outcomes in patients with hypertension taking chlorthalidone and hydrochlorothiazide.

Case study of a world hour record simulation in an elite cyclist: Insight into task failure.

The 'cycling hour-record' is one of the most prestigious events in cycling. However, little detailed analysis of such attempts is available. In preparation for a successful cycling hour-record attempt, an elite cyclist performed a full-hour simulation to provide insights into performance, physiological, aerodynamic and biomechanical limitations that could be identified in the preparation for a subsequent official attempt.