The functional activity of adult mouse Leydig cells in monolayer culture. Effect of lutropin and foetal calf serum.

Purified Leydig cells were obtained from adult mouse testes by mechanical dispersion followed by Percoll density-gradient centrifugation as described by Schumacher et al. (1978). The cells were then established in monolayer culture by maintaining them in medium and 10% serum at 32 degrees C in 95% O2, 5% CO2.

The heterogeneity of Leydig cells from mouse and rat testes--evidence for a Leydig cell cycle?

A method for purifying Leydig cells by centrifugation of testes cells on continuous density gradients of Percoll has been investigated. The distribution of Leydig cells in the separated bands of cells obtained and their receptor content and testosterone production after addition of lutropin (LH) has been measured. In agreement with previous work (Schumacher, Schäfer, Holstein & Hilz 1978) it was found that highly pure mouse Leydig cells (average density 1.

High-pressure liquid chromatography of steroids secreted by human adrenal and testis cells in monolayer culture.

Reversed-phase high-pressure liquid chromatography with gradient elution on Zorbax-ODS columns has been used to separate, identify, and measure, spectrophotometrically, the steroids secreted by both human adrenal and testis cells in primary monolayer culture. Three related systems using exponential concave gradients have been developed with the specific objective of resolving the steroids produced by these two tissues. A methanol-water gradient has been used to separate most adrenal steroids, an acetonitrile-water gradient to separate testis steroids, and a dioxane-water gradient to separate polar steroids, including aldosterone.