Biochemical and functional alterations in macrophages after thermal injury.

Biochemical and functional measurements of rat pulmonary alveolar macrophages were measured 4 h after 1 10-s, 26 to 28% total body surface area, full-thickness scald burn induced under ether anesthesia. Both phagocytic activity and capacity were significantly decreased to a comparable extent, whereas microbicidal activity was increased almost twofold in macrophages from the burned animals. Concurrent with the decreased phagocytic function was a marked impairment in chemotaxis and random migration of these cells when zymosan-activated serum was used as the chemoattractant.

The effects of essential fatty acid deficiency on pulmonary alveolar macrophage function.

Male rats were maintained for periods of up to 16 weeks on a fat free diet which was supplemented with either 4% tripalmitin (essential fatty acid [EFA] deficient) or with 4% safflower oil (SAFF, control). Pulmonary alveolar macrophages (PAM) were obtained by lung lavage. PAM from EFA deficient rats had reduced phagocytic activity and capacity.

Corynebacterium parvum treatment of P388 tumor-bearing mice. II. Lysosomal enzyme levels associated with P388 tumor cells.

The number of tumor cells recovered form the peritoneal cavity of mice administered Corynebacterium parvum (C. parvum), Bacillus Calmette-Guérin (BCG) or saline 2 days before tumor implant was assessed on days 1, 2, 3, 5 and 7 following the intraperitoneal (ip) administration of 10(6) P388 leukemic cells. C.

Corynebacterium parvum treatment of P388 tumor-bearing mice. I. Lysosomal enzyme levels in adherent peritoneal cells and peritoneal lavage fluid.

BDF1 mice treated with Corynebacterium parvum (C. parvum) 2 days before an implant of 106 P388 leukemic cells had up to an 110% increase in survival time above control; Bacillus Calmette-Guérin (BCG) treatment was ineffective. Acid phosphatase and beta-glucuronidase were measured in adherent peritoneal lavage cells and beta-glucuronidase in peritoneal lavage fluid form mice treated with C.

Enhancement of the antitumor-activity of Corynebacterium parvum by appropriate adjustment of dosage schedules.

Single and multiple doses of Corynebacterium parvum ranging from 0.01 to 60 mg/kg as the total dose were tested at selected times against 10(6) P388 leukemia cells inoculated ip in BDF1 mice. The minimum dose needed for maximum protection was determined according to the ability of the treatment to produce an increase in the lifespan (ILS) of mice.