Preparation of a Substrate for Studying Nucleosomes on Single Molecules of DNA.

DNA tightropes are a powerful single-molecule microscopy platform for the study of protein interactions with DNA on a single-molecule level. DNA molecules are suspended between two beads, granting full 3D access for a protein to bind and its interactions to be studied. Furthermore, tightropes can be custom-designed, permitting the study of protein interactions that are sequence- or substrate-dependent such as base mismatches or lesions.

Development of a collaborative learning health system data mart in pediatric liver transplantation.

Pediatric liver transplant is a lifesaving intervention for children with disparate pathology ranging from single-gene defects to global liver dysfunction and complications from progressive cirrhosis and portal hypertension. The Starzl Network for Excellence in Pediatric Transplantation (SNEPT), a novel learning health system dedicated to pediatric liver transplant, has previously identified practice variability, waitlist mortality, perioperative complications, and inadequate quality initiative implementation as critical barriers that deserve prioritization in the field. This project was a novel partnership between SNEPT and the United Network for Organ Sharing to co-design a pediatric transplant-specific data mart and web portal to systematically map an approach to understanding these barriers and deliver strategies to combat them.

Role models in gaming: Wishful identification and maladaptive daydreaming mediate the association between desire for fame and game engagement.

Background: The popularity of video games has considerably increased in recent years, with approximately 3.24 billion players worldwide in 2021. Empirical evidence suggests that the quality of engagement in video games can determine whether a gaming activity is associated with psychological distress (i.

Exploring the body condition of gray whales (Eschrichtius robustus) through adipocyte analysis during unusual mortality events.

The blubber is the characteristic fatty tissue of most marine mammals; it comprises adipocytes, blood vessels, lymphatics, and abundant collagen and elastic fibers. Containing the reserve of excess and accumulated energy from feeding. Previous studies found that the adipocyte area may change according to the amount of accumulated and expended energy during migration, reproduction, lactation and breeding.