Using a model of unilateral nephrectomy and contralateral heminephrectomy in weanling rats, we studied the effect of severe (6%) and moderate (14%) protein restriction on growth and renal function up to 24 wk of age. One-half the normal (N) and chronic renal insufficiency (CRI) rats underwent a cross-over protocol 6%----14% and 14%----6% at 10wk of age to analyze the contribution of early growth on ultimate wt and length. N or CRI fed a 6% protein diet were the smallest.
View Article and Find Full Text PDFTotal, packed cell and, plasma volume estimates were made for the whole body and selected tissues of rainbow trout by the simultaneous injection of radiolabelled trout erythrocyte ((51)Cr-RBC) and radioiodinated bovine serum albumin ((125)I-BSA) tracers. Blood volumes were estimated with both markers separately by the tracer-hematocrit method and as the combination of the(51)Cr-RBC packed cell and(125)I-BSA plasma volumes. Mean whole body blood volume was significantly less when calculated from the(51)Cr-RBC tracer data (3.
View Article and Find Full Text PDFA recent development in the preparation for amino acid analysis is the use of phenylisothiocyanate (PITC) as a precolumn derivatizing agent prior to analysis to form a stable derivative. These derivatives can then be separated by reversed phase high pressure liquid chromatography (HPLC). Because of interest in the accurate measurement of urinary hydroxyproline (HYP), PITC was used for derivatization followed by HPLC analysis.
View Article and Find Full Text PDFTaurine is the major free intracellular amino acid. It has become the focus of study by many as a conjugator of bile and as a neurotransmitter and intracellular messenger. In this report we document a technique for measuring taurine in physiologic samples which is rapid, reproducible, and accurate.
View Article and Find Full Text PDFComp Biochem Physiol A Comp Physiol
September 1987
1. Total blood volume and relative blood volumes in selected tissues were determined in non-anesthetized, confined rainbow trout by using 51Cr-labelled trout erythrocytes as a vascular space marker. 2.
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