Publications by authors named "R M Overmeer"
Background: The inability to predict treatment response of colorectal cancer patients results in unnecessary toxicity, decreased efficacy and survival. Response testing on patient-derived organoids (PDOs) is a promising biomarker for treatment efficacy. The aim of this study is to optimize PDO drug screening methods for correlation with patient response and explore the potential to predict responses to standard chemotherapies.
View Article and Find Full Text PDFArticle Synopsis
- Intestinal organoids created from LGR5 adult stem cells offer a more accurate and long-lasting model for studying human intestinal physiology compared to traditional models like Caco-2.
- These organoids were used to evaluate drug disposition, metabolism, and safety by conducting transport studies and assessing cell viability in response to toxic compounds.
- The findings indicate that organoids from different species mirrored in vivo safety profiles and displayed varying metabolic activities, suggesting their potential for drug testing and toxicity assessments.
Article Synopsis
- Patient-derived organoids (PDOs) are tiny lab-grown tissues that help scientists find new cancer treatments.
- Researchers tested 414 different drugs to see which ones could make cancer cells die instead of just stop growing.
- They found that a drug called vinorelbine works well against colorectal cancer cells and could be even better when combined with other treatments, leading to plans for patient tests.
Article Synopsis
- * The study focuses on drug screening with specific organoid types from head and neck and colorectal cancers, which can be used across various organoid models.
- * Methods include exposing organoids to drugs and measuring cell survival through ATP levels to determine sensitivity or resistance to treatments using metrics like IC50 and AUC.
Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells.
View Article and Find Full Text PDF