Validation of a radioimmunoassay procedure for N,N'-diacetylbenzidine, a metabolite of the chemical carcinogen benzidine, in urine.

The biaryl amine benzidine has been reported to produce tumors of the bladder in both man and animals. We have developed and validated a radioimmunoassay (RIA) procedure for monitoring potential human exposure to benzidine through detection in urine samples of one of its known urinary metabolites. The antibody used in the assay was produced in rabbits and is specific for N,N'-diacetylbenzidine (N,N'-DAB).

Development of a radioimmunoassay procedure for 4-acetamidobiphenyl, a metabolite of the chemical carcinogen 4-aminobiphenyl, in urine.

The synthetic chemical 4-aminobiphenyl (4-ABP) has been shown to be a bladder carcinogen in both man and animals. A valid radioimmunoassay for a metabolite of 4-ABP has been developed as a means to monitor potential human exposure to 4-ABP. The antibody was produced by the immunization of two female New Zealand White rabbits and was found to be highly specific for 4-acetamidobiphenyl (4-AABP), the acetylated metabolite of 4-ABP.

Use of a programmable desk-top calculator for the statistical quality control of radioimmunoassays.

We have developed an interactive statistical quality-control system for the small- to medium-sized radioimmunoassay laboratory, which can be used in a programmable desk-top calculator instead of the medium- or large-scale computer systems usually required. The design of this quality-control system is modeled after the suggestions of Rodbard and has three components. The first component evaluates the relationship between the measured response variable of the radioimmunoassay and the precision (or variance) of these measurements.