Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis.

Objective: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing.

Design: Prospective double-blinded study.

Setting: Private Clinic.

The dopamine receptor D2 genotype is associated with hyperprolactinemia.

Objective: To evaluate patients with hyperprolactinemia for the presence of dopamine receptor D2 polymorphisms.

Design: Case-control study.

Setting: Academic research environment.

Success rates following intracytoplasmic sperm injection are improved by using ZIFT vs IVF for embryo transfer.

Purpose: The purpose of this study was to analyze whether the mode of embryo transfer (ZIFT vs IVF) affected the outcome in intracytoplasmic sperm injection (ICSI) cycles.

Methods And Results: Eighty-two ICSI cycles (42 ZIFT and 40 IVF) were analyzed. Several variables, including patient age and weight, numbers of mature eggs collected, injected, and fertilized, fertilization rate, number of fertilized eggs obtained per cycle, numbers of zygotes/embryos transferred, clinical pregnancy rate, and implantation rate, were compared.

Laparoscopy: basic technique, instrumentation, and complications.

Laparoscopy has been an important diagnostic and therapeutic modality in gynecology for several decades. Recently, general surgeons and urologists have adopted laparoscopy to treat many disorders that formerly required an open laparotomy. Laparoscopic surgery requires additional instrumentation and skills as well as familiarity with a number of operative complications unique to laparoscopy.

Influence of growth factors in defined culture medium on in vitro development of mouse embryos.

Two-cell mouse embryos were cultured in Ham's F-10 medium (Gibco, Grand Island, NY) supplemented with various concentrations of transferrin, insulin, epidermal growth factor, platelet-derived growth factor, or fibroblast growth factor. Rates of development and the cell numbers per blastocyst were compared between embryos cultured in Ham's F-10 alone versus growth factor (GF) supplemented media. Rates of development in the presence of GF were inhibited significantly in some cases, and the cell numbers per blastocyst were identical in all groups.